![]() ![]() coli expression systems containing ColE1-type plasmids that confer ampicillin resistance. Each plasmid carries an origin of replication (ori) derived from pACYC and a chloramphenicol-resistance gene (Cm r) gene, which allows the use of E. Co-expression of a target protein with one of these five chaperone plasmids increases the recovery of soluble protein and minimizes product loss as a result of insoluble protein. The Chaperone Plasmid Set consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function together as a "chaperone team" to enable optimal protein expression and folding and to reduce protein misfolding. These plasmids also contain the necessary regulator ( araC or tet r) for each promoter. ![]() The chaperone genes are situated downstream of the araB or Pzt-1 (tet) promoters as a result, expression of target proteins and chaperones can be individually induced if the target gene is placed under the control of different promoters (e.g., lac). ![]()
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